The University of Sheffield
Browse
- No file added yet -

Kalb protein quantification test

Download (12.5 kB)
software
posted on 2018-02-26, 10:10 authored by Andrew Landels
This dataset/code forms part of Andrew Landels' thesis: "Improving proteomic methods and investigating H2 production in Synechocystis sp. PCC6803" http://etheses.whiterose.ac.uk/id/eprint/19034

A protein quantification method, devised by Kalb and Bernlohr, was utilised to remove the influence of phycobilisome pigments on protein quantification prior to analysis. Control samples of bovine serum albumin (BSA) and bovine cytochrome c (cyt) were used as standards of known concentration to investigate this method and how it scaled for individual proteins and mixtures of proteins.

This dataset shows that there was an over-estimation of cyt that scaled linearly, and a slight underestimation of BSA that did not appear to scale. When combined together, the effects of the linear scaling were still present, however were reduced by half.

Funding

EU FP7 308518

History

Ethics

  • There is no personal data or any that requires ethical approval

Policy

  • The data complies with the institution and funders' policies on access and sharing

Sharing and access restrictions

  • The data can be shared openly

Data description

  • The file formats are open or commonly used

Methodology, headings and units

  • Headings and units are explained in the files

Usage metrics

    Department of Chemical and Biological Engineering

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC