How the O2-dependent Mg-protoporphyrin monomethyl ester cyclase forms the fifth ring of chlorophylls
dataset
posted on 2021-01-29, 10:51 authored by Guangyu Chen, Nathan Adams, Philip JacksonPhilip Jackson, Mark DickmanMark Dickman, Christopher HunterChristopher HunterData-files acquired by reverse phase liquid chromatography coupled online to electrospray-ionisation mass spectrometry (Q Exactive HF, Thermo Scientific).<div>(1) 20200213_PJ_GC_MS1: full-scan MS, 500-700 m/z</div><div>(2) 20200213_PJ_GC_PRM: product ion scans by parallel reaction monitoring for cyclase substrate MgPME, product DV PChlide <i>a</i>, and catalytic intermediates 13<sup>1</sup>-hydroxy-MgPME and 13<sup>1</sup>-keto-MgPME at m/z 598, 611, 614 and 612 respectively.</div><div><p>Thermo RAW files are included in mzML
format which is readable by open source applications such as SeeMS
(http://proteowizard.sourceforge.net/download.html).</p></div>
Funding
Engineering new capacities for solar energy utilisation in bacteria
Biotechnology and Biological Sciences Research Council
Find out more...Mass spectrometry underpinning synthetic biology, industrial biotechnology and world class bioscience
Biotechnology and Biological Sciences Research Council
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