Innovative P(3HB)/Carbon Based Material Composites: Promising Materials for Bone Tissue Engineering - Raw data

<p dir="ltr">Bone tissue engineering (BTE) has emerged as a promising field to address current limitations in treating bone defects with critical gap sizes. However, there is still a need for innovative solutions that can produce bone substitutes with suitable mechanical properties while also being bioactive <i>in-vivo</i>. Poly(3-hydroxybutyrate) P(3HB), a natural and sustainable polymer from the polyhydroxyalkanoate family, has been previously explored for BTE. Nevertheless, its poor mechanical properties and thermal instability remain significant bottlenecks. In this study, P(3HB) was reinforced with various carbon-black materials (CBMs) to evaluate the effects of aging on its thermal, structural, and mechanical properties. After assessing the mechanical and thermal properties of the CBM/P(3HB) composites, the interaction of the fillers with the polymeric matrix and their spatial distribution were examined using advanced imaging techniques, including Atomic Force Microscopy (AFM), Secondary Electron Hyperspectral Imaging (SEHI), and Short-Wave Infrared (SWIR) analysis. To evaluate biocompatibility, the cytotoxicity of CBM/P(3HB) composites was tested by measuring metabolic activity and live/dead cell ratios of MG63 cells and primary human osteoblasts. Furthermore, the osteogenic potential of the composites was assessed through alkaline phosphatase (ALP) expression and the formation of mineralized nodules in primary human osteoblasts. From these analyses, P(3HB)/inkjet emerged as a novel CBM/P(3HB) material with strong potential as a bone substitute for BTE applications.</p><p dir="ltr">The data has been separated into FTIR and cell culture. The cell culture data is further subdivided into MG63 cells and human primary osteoblasts. Each cell type data contains resazurin assay, ALP and live/dead for aged and fresh composite films.</p>