These .zip files contain the raw data associated with the manuscript 'Bacteria solve the problem of crowding by moving slowly' (Meacock, O.J., Doostmohammadi, A., Yeomans, J.M., Foster, K.R., Durham, W.M., 2020). The contents consists of the following folders:
-Cell morphology data: Data used to measure the length of WT and pilH cells at exponential-phase in liquid culture and in the monolayer. Used in Extended Data Fig. 6a.
-Liquid culture competition CFUs: Spreadsheet containing the raw CFU counts associated with Extended Data Fig. 8b.
-Low-density cell tracks: Tracks used to measure motility and shape of strains under low density subsurface conditions. Used in Extended Data Figs. 1c, 3b, 6a, 9d.
-Monolayer cell and defect tracks: Defect and cell tracks from high-density WT and pilH subsurface monolayers. Used in Figs. 1g, 3e-f, Extended Data Figs. 3a and 4.
-Raw confocal microscopy data: Raw confocal imaging files of surficial and subsurficial colonies. Used in Fig. 1d, Extended Data Fig. 2a.
-Raw edge composition snapshots: Raw images used to generate Fig. 2g, Extended Data Fig. 10c-e.
-Raw high-resolution rosette data: Raw and partially processed imaging data associated with the rosettes presented in Fig. 4d-f and Extended Data Fig. 7.
-Subsurface colony composition and packing fraction data: Spreadsheets containing measurements of colony edge position, edge population composition and edge packing fraction, used to generate Fig. 2c-e, Extended Data Figs. 9b,c, 10a,b.
-Subsurface colony edge coordinates: Spreadsheets containing measurements of colony edge position, used to generate Fig. 1f,g, Extended Data Fig. 1a,b.
-Surficial colony composition measurements: Raw CFU counts and images used to generate Fig. 2a,b.
For further details on the format of individual files, please refer to the readme.txt files in each folder.
Funding
Life Sciences Interface Doctoral Training Centre
Engineering and Physical Sciences Research Council