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Readme.txt (1.83 kB)
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Monolayer cell and defect tracks.zip (1.01 GB)
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Raw confocal microscopy data.zip (125.58 MB)
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Raw edge composition snapshots.zip (272.69 MB)
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Raw high-resolution rosette data.zip (510.86 MB)
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Subsurface colony composition and packing fraction data.zip (8.57 MB)
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Subsurface colony edge coordinates.zip (1.31 MB)
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Surficial colony composition measurements.zip (12.6 MB)
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Cell morphology data.zip (10.75 MB)
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Liquid culture competition CFUs.zip (9.92 kB)
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Low-density cell tracks.zip (62.81 MB)
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11 files

Raw data for 'Bacteria solve the problem of crowding by moving slowly'

dataset
posted on 2020-08-11, 21:22 authored by Oliver MeacockOliver Meacock, William DurhamWilliam Durham
These .zip files contain the raw data associated with the manuscript 'Bacteria solve the problem of crowding by moving slowly' (Meacock, O.J., Doostmohammadi, A., Yeomans, J.M., Foster, K.R., Durham, W.M., 2020). The contents consists of the following folders:

-Cell morphology data: Data used to measure the length of WT and pilH cells at exponential-phase in liquid culture and in the monolayer. Used in Extended Data Fig. 6a.
-Liquid culture competition CFUs: Spreadsheet containing the raw CFU counts associated with Extended Data Fig. 8b.
-Low-density cell tracks: Tracks used to measure motility and shape of strains under low density subsurface conditions. Used in Extended Data Figs. 1c, 3b, 6a, 9d.
-Monolayer cell and defect tracks: Defect and cell tracks from high-density WT and pilH subsurface monolayers. Used in Figs. 1g, 3e-f, Extended Data Figs. 3a and 4.
-Raw confocal microscopy data: Raw confocal imaging files of surficial and subsurficial colonies. Used in Fig. 1d, Extended Data Fig. 2a.
-Raw edge composition snapshots: Raw images used to generate Fig. 2g, Extended Data Fig. 10c-e.
-Raw high-resolution rosette data: Raw and partially processed imaging data associated with the rosettes presented in Fig. 4d-f and Extended Data Fig. 7.
-Subsurface colony composition and packing fraction data: Spreadsheets containing measurements of colony edge position, edge population composition and edge packing fraction, used to generate Fig. 2c-e, Extended Data Figs. 9b,c, 10a,b.
-Subsurface colony edge coordinates: Spreadsheets containing measurements of colony edge position, used to generate Fig. 1f,g, Extended Data Fig. 1a,b.
-Surficial colony composition measurements: Raw CFU counts and images used to generate Fig. 2a,b.

For further details on the format of individual files, please refer to the readme.txt files in each folder.

Funding

Life Sciences Interface Doctoral Training Centre

Engineering and Physical Sciences Research Council

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How do bacteria sense and navigate chemical gradients within biofilms?

Biotechnology and Biological Sciences Research Council

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Sheffield antimicrobial resistance network - SHAMROK

Engineering and Physical Sciences Research Council

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Understanding and engineering complex microbial communities

Wellcome Trust

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The Evolution of Bacterial Warfare

European Research Council

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History

Ethics

  • There is no personal data or any that requires ethical approval

Policy

  • The data complies with the institution and funders' policies on access and sharing

Sharing and access restrictions

  • The data can be shared openly

Data description

  • The file formats are open or commonly used

Methodology, headings and units

  • Headings and units are explained in the files