Data for paper "A new mode of contrast in biological second harmonic generation microscopy"
dataset
posted on 2017-10-06, 09:20 authored by Nicola GreenNicola Green, Robin M Delaine-Smitth, Hannah J Askew, Robert Byers, Gwendolen ReillyGwendolen Reilly, Stephen MatcherStephen Matcher<p>Enhanced image contrast in biological
second harmonic imaging microscopy (SHIM) has previously been reported via
quantitative assessments of forward- to epi-generated signal intensity ratio
and by polarization analysis. Here we demonstrate a new form of contrast: the
material-specific, wavelength-dependence of epi-generated second harmonic
generation (SHG) excitation efficiency, and discriminate collagen and myosin by
ratiometric epi-generated SHG images at 920 nm and 860 nm. </p>
<p>Collagen shows increased SHG
intensity at 920 nm, while little difference is detected between the two for
myosin; allowing SHIM to characterize different SHG-generating components
within a complex biological sample.</p><p><br></p><p>Scientific Reports 7(1) Article number:13331 (2017)</p>
<p>DOI: 10.1038/s41598-017-13752-y</p>
Funding
EPSRC grants EP/I018328/1 & EP/K009699/1 and the University of Sheffield Women Academic Returners Program.
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