The bacterial cell envelope, in particular the cell wall, is considered
the main controlling factor in the biosorption of aqueous uranium (VI)
by microorganisms. However, the specific roles of the cell wall,
associated biomolecules, and other components of the cell envelope are
not well defined. Here we report findings on the biosorption of uranium
by isolated cell envelope components and associated biomolecules, with
P. putida 33015 and B. subtilis 168 investigated as representative
strains for the differences in Gram-negative and Gram-positive cell
envelope architecture, respectively. The cell wall and cell surface
membrane were isolated from intact cells using a French Pressure Cell
and characterised by X-Ray Photoelectron Spectroscopy (XPS) and
Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FT-IR)
spectroscopy. Uranium biosorption was investigated as a function of cell
envelope component and pH, comparing with intact cells and was
quantified by ICP-OES.
Supplementary information includes characterisation of XPS spectra, uranium speciation, additional adsorption isotherms, langmuir fittings and detailed FT-IR characterisation of cell envelope components.
Data includes raw XPS and FT-IR spectra, peak identifications and uranium isotherm fittings from data collected by ICP-OES.
Hard-soft matter interfaces: from understanding to engineering
Engineering and Physical Sciences Research Council